BioScope Resolve. The Most Advanced AFM for Life Science Research. Andrea Slade, PhD. Senior Life Sciences Applications Scientist. JSPM Instructional Manual · SPM Training Manual – Bioscope II · SPM Training Manual – Multimode · User Manual for Electrostatic Force Microscopy. Magnetic Force Microscopy, and this manual is designed to serve as a supplement to All SPM Lab users must log on to the Bioscope II first in order to turn the.
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The nominal force applied to the surface can be calculated from the force equation. A simple feedback loop emerges in this analogy: The Y positioner knob moves the beam perpendicular to the major axis of the cantilever substrate. Other suggested parameter values are listed manua Section 8. Note that this will result in an image that shows only large transitions in Z; this is normal.
The detector adjustment knobs are at the left side of the head Figure manuak. Initially, set Proportional gain to 1.
BioScope™ Instruction Manual
D Align holes at the front and rear of the baseplate with tapped holes in the microscope frame. Whenever major scan parameters are changed e. Baseplate Slider Even Spacing Figure 3. For example, although Height data can be displayed for most types of imagery, only Msnual displays Amplitude data. Pre-Imaging Preparation Cantilever Preparation the opposite side from the cantilevers.
If the Initialize command has just been run, there should be ample clearance, unless the sample is unusually biosckpe. Plug the vacuum pump in and switch on. The manual also contains a tab-indexed table of contents to reduce searching times. Scan rates of 1. Do not attempt to set Interleave mode to Enabled at this point.
Center the AFM cantilever with the microscope optics Using a low-power objective lens e. While holding down the next substrate on the strip with the wooden end of a cotton swab, grip the overhanging piece with a pair of wide tweezers and rotate mabual.
In these instances, LookAhead gain can be maximized. Refer to Figure 3. Step 3 provides instructions on how to separate substrates from strips.
BioScope™ Instruction Manual |
The Cantilever Tune panel allows the operator to tune in either of two ways: The brief contact between the cantilever and sample reduces the amplitude of the cantilever oscillation. Disabled Lift start height: The left side of the graph is equal to the Z scan start voltage while the right side of the screen is equal to the quantity Z scan start minus Z scan size.
To do this, the cantilever’s support is moved up and down on the end of a piezoelectric tube. Lateral movements of the beam are also monitored, corresponding to frictional phenomena on the surface. At point 5, the spring force of the cantilever equals the attractive forces between the tip and the sample. Also attach two pads to the mating surfaces on the bottom of the stage plate. Tuning the Cantilever Drive Frequency The Drive frequency selected to oscillate the cantilever plays an important role in the performance of the microscope while in TappingMode.
Another way is to use the Force Calibration command to select the Setpoint and estimate the contact force, as described in Chapter 6. C Use the supplied Allen wrench to gently rotate the cam at the front-center of the stage plate; this can be used to manipulate the stage plate when aligning the SPM tip with the microscope objective. Piezo oscillations typically cause high frequency wavy lines in the Real-time image.
Unfortunately, this arrangement did not work well for rougher surfaces: Side margin icons are used within some chapters to divide information according to image type TappingMode, contact AFM, etc. Raise the gains using the arrow keys until the input voltage is minimized.
Important to BioScope Users! The scanner houses the piezoelectric transducer. This sequence may often be necessary when engaging wet samples.
They are robust and relatively inexpensive. For example, if the setpoint is set to Here are a few examples of specialized probes: To optimize the gains for Height data, increase the Integral gain until the piezo begins to oscillate, then eliminate the oscillations by reducing the gain with two or three clicks of the left-arrow key. Chapters 6—13 cover all basic imaging techniques performed by the BioScope.
However, the operating point can be changed after the cantilever has been engaged. Optimization of Scanning Parameters Careful selection of the scan parameters is important to the successful application of contact AFM. The harder the tip is pressed into the sample, the more RMS oscillation is reduced.
Verify that Slow scan axis is Enabled otherwise, streaks may appear on the screen. Clean sample holder Carefully clean the surface of the vacuum sample holder.
In the following examples, the nominal parameter values may vary depending upon the actual cantilever used. Introduction Attach the precision X-Y manipulator to Baseplate Bolt the precision X-Y manipulator to the baseplate vioscope three knurled thumb screws located on the right-underside of the baseplate.
After using Withdraw to retract the tip, simply position the next sample on the stage, then click Automatic. Introduction Chapters 12 and 13 are complementary and should be read together.