PDF | Gerbera (Gerbera jamesonii Bolus) is one of the most popular ornamental flowers worldwide and used both as cut flower and potted. PDF | Gerbera jamesonii (gerbera) is an important cut-flower in the global floricultural industry. Micropropagation is the main system used to. PDF | In present research, the effects of light quality on micropropagation of gerbera were investigated. The MS medium containing 1 mg L-1 BAP and mg .
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Percentage of callus and root formation per explants produced on MS medium supplemented with different hormones and concentration after 8 weeks in culture formed in these experiments were micropropagatino green. In vitro Culture of Higher Plants.
Factors affecting differentiation in vitro and mass propagation scheme for Begonia x hiemalis. This species consists of many cultivars with variety of colors and shapes and they are popular commercial plants. In the present study, experiments gerberz conducted to investigate organogenesis from various sources of Gerbera explants. Survival rate of micro propagated plants that were transferred to soil were investigated Fig. Micropropagation of Woody Plants, Ahuja, M.
Direct shoot regeneration from lamina explants of two commercial cut flower cultivars of Anthurium andraeanum Hort. Bigot reported that the addition of g L -1 activated charcoal in the culture medium showed vigorous rooting from excised shoots of Begoniax hiemalis. Formation of callus from explants tissues involves the development of progressively more random planes of cell division, less frequent specialization of cells and loss kicropropagation organized structures Thorpe, ; Wagley et al.
However, in Gerberaaddition of charcoal is not necessary for rooting. Plantlets were transferred to soil and maintained in culture room for weeks for adaptation process before being transferred to field environment. The physiological status of the donor is determined by environmental conditions such as temperature, light intensity, day-length and light wavelength.
Regenerated plants were rooted on Murashige and Skoog getbera medium. Different types of auxin and cytokinin combinations were used in order to obtain complete regeneration of Gerbera in vitro.
Rancillac terbera Nourrisseau improved the performance of micropropagated strawberry plants by decreasing the cytokinin concentration and limiting the number of subcultures. Takayama and Misawa reported that medium containing 0. Growth Optimization and Organogenesis of Gerbera jamesonii Bolus ex.
MICROPROPAGATION OF GERBERA | ICAR-Indian Institute of Horticultural Research
High efficiency adventitious shoot bud formation and plant regeneration from leaf explants of Dianthus chinensis L. The authors wish to thank the Institute of Biological Sciences and University of Malaya, Kuala Lumpur, Malaysia for providing all facilities and financial support.
Abstract Regeneration potentials in Gerbera jamesonii Bolus ex. Effects of growth regulators and incubation period on in vitro regeneration of adventitious shoots from Gerbera petioles. Shoots were obtained after 28 days of culture and shoot growth was normal. Highest root formation was obtained when leaf explants were cultured on MS medium supplemented with 0. Root explants itself also induced the formation of root in vitro.
Numerous types of explants and protocols for micropropagation have been established and used for gerbera.
The studing of micropropagation of Gerbera sp via shoot tip explant.
Gerber of ornamental plants-cut flowers. This research was funded by Vote F Grant No. Relatively, in this experiment, callus formation was optimum when leaf explant micropropavation cultured on MS medium supplemented with 1. Epigenetic variations in micropropagated gerbera are frequently observed only with high concentrations of cytokinins in the culture medium but somaclonal variation is rare.
Adventitious micrporopagation were obtained from petiole explants cultured on Murashige and Skoog MS medium supplemented with 2.
Percentage of shoot formation and number of shoots per explants produced on MS medium supplemented with different hormones and concentrations after 8 weeks in culture. Adventitious organogenesis or shoot formation is a preferred system as it enables to retain the clonal fidelity since many ornamental species are cultivars that are propagated for one or more unique characteristics Kantia and Kothari, Micropropagation is the main system used to clonally propagate gerbera in vitro resulting in the production of millions of plantlets each year.
The main purpose for propagation of ornamental plants is for its aesthetic value. Root micropropagztion occurred when explants were cultured on medium with higher auxin concentration and lower cytokinin concentration. Micropropagation selected somaclones Begonia Saintpaulia J.
MICROPROPAGATION OF GERBERA
Various explants have been used for direct shoot formation. Highest numbers of shoots from petiole explants 9. These plant growth regulators were added to Murashige and Skoog medium in different combinations and concentrations. Gerbera jamesonii Bolus ex. BAP strongly enhanced regeneration of shoots in petiole explants of Gerbera jamesonii.